Clinical Research Groups at the Department of Dermatology

 

Department of Dermatology
Medical and Health Science Center
University of Debrecen
Director: Éva Remenyik, MD, PhD, DSc
e-mail (main office): dermatologia[at]med.unideb.hu
http://dermatology.med.unideb.hu

 

Research background:
Research groups at the Department of Dermatology, Medical and Health Science Center, University of Debrecen are groups of resident scientist, PhD students and clinicians conducting research projects on site and in collaboration with other DERMINOVA members. Their research are oriented to certain dermal pathologic conditions such as UV damaged skin, psoriasis, urticaria, chornic wounds, melanoma & other skin cancers. Unique feature of their research that the subject of examination is mostly arising from clinical patients' samples like blood, other body fluids and biopsy specimens. This clinical experimental setting confirms the validity of experimental data obtained from in vitro and animal models of local and collaborating scientists. This activity is based on accessible patients from the Department's outpatient clinics to be involved as medical scientific research target or as a biological sample donor. The local laboratory capacities - like laboratory of dermatohistology, laboratory of molecular biology, cell culture laboratory and laboratory of experimental animal surgery with SCID mice- are also supporting our scientific goals in the DERMINOVA project.
 

Research area:
Photodermatology, dermatoimmunology, oncodermatology, dermatologic surgery, epidemiology
 

Photodermatology research group:
Focus of the fotodermatology research group is the pathomechanism of the polymorphic light exhantem (PLE). Experimental targets are the UVB induced expressional changes of defensins, inflammatory cytokines and the excision repair capacity in PLE patiens. The investigation of the role of CPD photoproducts and their remaining mutations after UVB treatment in the expressional change of genes, patways in keratinocytes, mentioned above.
 

UV irradiated human skin (H&E, frozen section) CPD phtoprodutcs in UVB irradiated humasn skin, (IHC with DAB of frozen section)

Members:

Éva Remenyik, MD, PhD, DSc
PI of the photodermatology research group,
full professor,
head of the Department of Dermatology
remenyik[at]med.unideb.hu
  Gabriella Emri, MD, PhD
senior lecturer, dermatologist,
member of the photodermatology research group
 
gemri[at]med.unideb.hu
  Dávid Rózsa
PhD student, biologist,
member of the photodermatology research group
 
drozsa[at]med.unideb.hu
  Eszter Emri
PhD student, biochemist,
member of the photodermatology research group
emrie[at]med.unideb.hu

 
Attila Balogh
graduating PhD student, biologist,
member of the photodermatology group
dermatologia[at]med.unideb.hu

 

Publications:

Representative publications:
Katona E, Aslanidis C, Remenyik E, Csikós M, Kárpáti S, Paragh G, Schmitz G. Identification of a novel deletion in the ABCC6 gene leading to Pseudoxanthoma elasticum. J Dermatol Sci. 2005 Nov;40(2):115-21. Epub 2005 Sep 23. PubMed PMID: 16183260.

Remenyik E, Wikonkál NM, Zhang W, Paliwal V, Brash DE. Antigen-specific immunity does not mediate acute regression of UVB-induced p53-mutant clones. Oncogene. 2003 Sep 25;22(41):6369-76. PubMed PMID: 14508517.

Wikonkal NM, Remenyik E, Knezevic D, Zhang W, Liu M, Zhao H, Berton TR, Johnson DG, Brash DE. Inactivating E2f1 reverts apoptosis resistance and cancer sensitivity in Trp53-deficient mice. Nat Cell Biol. 2003 Jul;5(7):655-60. PubMed PMID: 12833065.

Brash DE, Wikonkal NM, Remenyik E, van der Horst GT, Friedberg EC, Cheo DL, van Steeg H, Westerman A, van Kranen HJ. The DNA damage signal for Mdm2 regulation, Trp53 induction, and sunburn cell formation in vivo originates from actively transcribed genes. J Invest Dermatol. 2001 Nov;117(5):1234-40. PubMed PMID: 11710938.

Zhang W, Remenyik E, Zelterman D, Brash DE, Wikonkal NM. Escaping the stem cell compartment: sustained UVB exposure allows p53-mutant keratinocytes to colonize adjacent epidermal proliferating units without incurring additional mutations. Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13948-53. Epub 2001 Nov PubMed PMID: 11707578; PubMed Central PMCID: PMC61147.
 

Recent publications:
Paragh G, Ugocsai P, Vogt T, Schling P, Kel AE, Tarabin V, Liebisch G, Orsó E,Markó L, Balogh A, Köbling T, Remenyik E, Wikonkál NM, Mandl J, Farwick M, Schmitz G. Whole genome transcriptional profiling identifies novel differentiation regulated genes in keratinocytes. Exp Dermatol. 2009 Dec 2. [Epubahead of print] PubMed PMID: 19961536.

Balogh A, Paragh G Jr, Juhász A, Köbling T, Törocsik D, Mikó E, Varga V, Emri G, Horkay I, Scholtz B, Remenyik E. Reference genes for quantitative real time PCR in UVB irradiated keratinocytes. J Photochem Photobiol B. 2008 Dec 11;93(3):133-9. Epub 2008 Aug 14. PubMed PMID: 18789713.

Remenyik E, Lecha M, Badenas C, Kószó F, Vass V, Herrero C, Varga V, Emri G, Balogh A, Horkay I. Childhood-onset mild cutaneous porphyria with compound heterozygotic mutations in the uroporphyrinogen decarboxylase gene. Clin Exp Dermatol. 2008 Aug;33(5):602-5. Epub 2008 May 6. PubMed PMID: 18462440.

Horkay I, Emri G, Varga V, Simics E, Remenyik E. Photosensitivity skin disorders in childhood. Photodermatol Photoimmunol Photomed. 2008 Apr;24(2):56-60. Review. PubMed PMID: 18353083.

Emri G, Horkay I, Remenyik E. [The role of free radicals in the UV-induced skin damage. Photo-aging]. Orv Hetil. 2006 Apr 23;147(16):731-5. Review. Hungarian. PubMed PMID: 16711258
 

Dermatoimmunoogy research group:

The dermatoimmunology research group conducts examinations about the function and alterations of TH1-TH2-TH17, TREG, NKT, APC activity in patients with atopic dematitis, urticaria and psoriasis. The role of cutaneous D-vitamin physiology in psoriasis is also a main interest of the group

Scope of the dermatoimmunology group

The dermatooncology & dermatologic surgery group is currently conducting research about the role of PARP activation in wound healing of chronic ulcers.

Members:

Andrea Szegedi, MD, PhD, DSc
associate professor, dermatologist
PI of the dermatoimmunology research group,
head of the Subdepartment of Dermatoimmunology
 
aszegedi[at]med.unideb.hu
  Krisztián Gáspár MD
assistant lecturer, dermatologist
PhD student,
member of the dermatoimmonology reseach group
nurk7[at]freemail.hu
  Georgina Nagy
graduating PhD student, biologist
member of the dermatoimmonology reseach group
dermatologia[at]med.unideb.hu

 

Publications:

Representative publications:
Aleksza M, Irinyi B, Lukács A, Antal-Szalmás P, Hunyadi J, Szegedi A.: Increased frequency of intracellular IL-13 and IL-10, but not IL-4 expressing CD4+ and CD8+ peripheral T cells of patients with atopic dermatitis. Br. J. Dermatol. 2002: 147: 1135-1141.

Gyimesi E, Sipka S, Dankó K, Kiss E, Hídvégi B, Gál M, Hunyadi J, Irinyi B, Szegedi A.: Basophil CD63 expression assay on highly sensitised atopic donor leukocytes- a useful method in chronic autoimmune urticaria. Br. J. Dermatol. 2004: 151: 388-396.

Aleksza M, Szegedi A, Antal-Szalmás P, Irinyi B, Gergely L, Ponyi A, Hunyadi J, Sipka S, Zeher M, Szegedi Gy, Dankó K.: Altered cytokine expression of whole blood Thelper and Tcytotoxic lymphocytes of patients suffering from polymyositis and dermatomyositis. Ann Rheum Dis. 2005: 64: 1485-1489.

Szegedi A, Simics E, Aleksza M, Horkay I, Gaál K, Sipka S, Hunyadi J, Kiss E.: Ultraviolet-A1 Phototherapy Modulates Th1/Th2 and Tc1/Tc2 Balance in Patients with Systemic Lupus Erythematosus. Rheumatology 2005: 44: 925-931.

Szegedi A, Irinyi B, Gál M, Hunyadi J, Dankó K, Kiss E, Sipka S, Gyimesi E.: Significant correlation between the CD63 assay and the histamine release assay in chronic urticaria. Br. J. Dermatol. 2006: 155: 1. 67-75.

Recent publications:
Sümegi A, Szegedi A, Irinyi B, Gaál M, Hunyadi J, Antal-Szalmás P.: Altered serum concentration and altered expression of the components of the CD14/TLR complex on the peripheral leukocytes of patients with atopic dermatitis. Int Arch Allergy Immunol. 2007: 143: 3. 177-84.

Irinyi B, Széles Gy, Gyimesi E, Tumpek J, Ádány R, Herédi E, Hunyadi J, Szegedi A.: Clinical and laboratory investigations in chronic urticaria patients. Int Arch Allergy Immunol. 2007: 144: 3. 217-225.

Szegedi A, Páyer E, Czifra G, Tóth BI, Schmidt E, Kovács L, Blumberg PM, Bíró T: Protein kinase C isoenzymes differentially regulate the differentiation-dependent expression of adhesion molecules in human epidermal HaCaT keratinocytes. Exp Dermatol, 2009: 18: 2. 122-9.

Gaspar K, Kukova G, Bunemann E, Sonkoly E, Muller A, Lauerma AI, Alenius H, Kemeny L, Dieu-Nosjean MC, Fischer JW, Ruzicka T, Zlotnik A, Homey B: The chemokine receptor CCR3 participates in tissue remodeling during atopic skin inflammation.
J Invest Dermatol 2008; 128: S185
 

Dermatologic surgery research group:
The research group is currently conducting research about the role of PARP activation in wound healing of chronic ulcers.

SCID mouse transplanted with human tumo

a) SCID mouse

b) Human xenotransplant tumor on SCID muose 

 

c) Full thickness human skin transpalnnted on SCID mouse

 

d) Hystopathologyc confirmation of c), no sign of inflammation or rejection

Members:

  István Juhász MD, PhD
PI of dermatologic surgery research group,
associate professor, dermatologist
head of the Subdepartent of Dermatosurgery
ji[at]med.unideb.hu
  Éva Szabó MD PhD
senior lecturer, dermatologist
member of the dermatologic surgery group
eszabo[at]med.unideb.hu
  Levente Lukács DD
graduating PhD student, dentist,
member of the dermatologic surgery group
drlukacslevente[at]yahoo.com

 

Publications:
Representative publications:
Juhász I, Simon M Jr, Herlyn M, Hunyadi J.:Repopulation of Langerhans cells during wound healing in an experimental human skin/SCID mouse model. Immunol Lett. 1996 Sep;52(2-3):125-8.

Simon M Jr, Juhász I, Herlyn M, Hunyadi J. Thrombospondin receptor (CD36) expression of human keratinocytes during wound healing in a SCID mouse/human skin repair model. J Dermatol. 1996 May;23(5):305-9. PubMed PMID: 8675818.

Juhasz I, Albelda SM, Elder DE, Murphy GF, Adachi K, Herlyn D, Valyi-Nagy IT, Herlyn M. Growth and invasion of human melanomas in human skin grafted to immunodeficient mice. Am J Pathol. 1993 Aug;143(2):528-37. PubMed PMID: 8342600; PubMed Central PMCID: PMC1887031.

Juhasz I, Lazarus GS, Murphy GF, Shih IM, Herlyn M. Development of pemphigus vulgaris-like lesions in severe combined immunodeficiency disease mice reconstituted with lymphocytes from patients. J Clin Invest. 1993 Nov;92(5):2401-7. PubMed PMID: 8227357; PubMed Central PMCID: PMC288423.

Virág L, Szabó E, Gergely P, Szabó C. Peroxynitrite-induced cytotoxicity:mechanism and opportunities for intervention. Toxicol Lett. 2003 Apr 11;140-141:113-24. Review. PubMed PMID: 12676457..

Recent publications:
Kiss B, Bíró T, Czifra G, Tóth BI, Kertész Z, Szikszai Z, Kiss AZ, Juhász I, Zouboulis CC, Hunyadi J. Investigation of micronized titanium dioxide penetration in human skin xenografts and its effect on cellular functions of human skin-derived cells. Exp Dermatol. 2008 Aug;17(8):659-67. Epub 2008 Jun 28. PubMed PMID: 18312389.

Nagy T, Glavinas H, Szincsák N, Hunyadi J, Jánossy T, Duda E, Vizler C, Juhász I. Tumor cells expressing membrane-bound tumor necrosis factor activate macrophages and have a compromised growth in immunosuppressed and immunodeficient mice. Cancer Lett. 2003 Jun 30;196(1):49-56. PubMed PMID: 12860289.

Szincsák N, Hegyesi H, Hunyadi J, Falus A, Juhász I. Different h2 receptor antihistamines dissimilarly retard the growth of xenografted human melanoma cells in immunodeficient mice. Cell Biol Int. 2002;26(9):833-6. PubMed PMID: 12377215.

Szincsák N, Hegyesi H, Hunyadi J, Martin G, Lázár-Molnár E, Kovács P, Rivera E, Falus A, Juhász I. Cimetidine and a tamoxifen derivate reduce tumour formation in SCID mice xenotransplanted with a human melanoma cell line. Melanoma Res. 2002 Jun;12(3):231-40. PubMed PMID: 12140379.

Bai P*, Hegedűs Cs*, Szabó É*, Gyüre L, Bakondi E, Brunyánszki A, Gergely S, Szabó C and Virág L: Poly(ADP-ribose) polymerase mediates inflammation in a mouse model of contact hypersensitivity. J. Invest. Dermatol. 2008 Jul 17. PMID: 18633442 * shared first authorship