Cell Analysis Core Facility / János Szöllősi

Personal data: 

János Szöllősi
Ph.D., D.Sc.
E-mail: szollo[at]med.unideb.hu
http://www.biophys.dote.hu

Services: 

The Cell Analysis Core Facility houses a variety of advanced microscopic imaging and flow cytometric instruments, which are available for users from the university and other institutes.

Infrastucture: 

Olympus FV 1000 confocal laser scanning microscope equipped with fluorescence correlation/crosscorrelation spectroscope (FCS, FCCS). Modalities: confocal 2D/line/point scanning, DIC, FCS/FCCS, spectral imaging, fluorescence recovery after photobleaching (FRAP), alternative excitation fluorescence resonance energy transfer measurement (ALEX FRET) with single molecule sensitivity. TIRF/PALM upgrade is in progress. Lasers lines: 458, 477, 488, 514 543,633 nm.

Zeiss LSM 510 – Confocor 2 confocal laser scanning microscope equipped with two-channel fluorescence correlation/crosscorrelation spectroscope. Modalities: confocal 2D/line/point scanning, DIC, FCS/FCCS, spectral imaging with META detector, FRET, FRAP. Laser lines: 351, 364, 458, 477, 488, 514 543,633 nm.

iCys laser scanning cytometer
Based on an Olympus BX inverted fluorescence microscope with digital imaging. Capable of light scattering and fluorescence measurements on slides or cell culture plates, re-localization of cells. Laser lines: 405, 488, 633 nm.

Becton Dickinson FACS DiVa flow cytometer and cell sorter
Fluorescence based sorting and measurement of fluorescently stained cells/microbeads. Sorting rate is 5000 cells per seconds. 3 lasers (351, 364, 488, 514, 532, 633 nm), forward and side scattering, 6 fluorescence detection channels, fluorescence anisotropy. Digital data acquisition with 16-bit detection (five-decade linear dynamic range from 0 to 262,000).

Becton Dickinson FACS Array high speed bioanalyzer
Compact, easy-to-operate instrument. Capable of fluorescence and light scattering measurements on cell suspensions or microbeads conjugated with biomolecules (cytokine bead assay) in 96-well plates. 2 lasers (532, 635 nm), 2 scattering and 4 fluorescence detection channels. Digital data acquisition with 16-bit detection.

Atomic force microscope
Stand-alone atomic force microscope (AFM) mounted on a Zeiss inverted fluorescence microscope. Fluorescence mode allows detection of cell surface antigen distribution. For AFM tapping and contact modes are available.

Methods: 

Confocal laser scanning microscopy, flow cytometric and microscopic fluorescence resonance energy transfer methods with cell-by-cell, pixel-by-pixel and single molecule resolution, fluorescence correlation and crosscorrelation spectroscopy to detect molecular mobility and co-mobility with single molecule sensitivity, fluorescence recovery after photobleaching, laser scanning cytometry alloying high throughput and morphological information. Atomic force microscopy to detect topography of cell/tissue surface.

 

Contact: 


 Dr. György Vámosi vamosig[at]med.unideb.hu
 Dr. György Vereb vereb[at]med.unideb.hu