Proteomics Core facilities / József Tőzsér

József Tőzsér
Ph.D., D.Sc.
E-mail: tozser[at]med.unideb.hu
http://bmbi.med.unideb.hu/proteomics

 

Services: 

• Protein molecular weight determination
• Protein identification
• Protein post-translational modification detection
• Protein quantitation using SILAC or iTRAQ methods
• MS data analysis using MASCOT, ProID or ProteinPilot softwares
• Separation of proteins using 1D or 2D electrophoresis
• Proteome comparison using 2D electrophoresis
• Gel imaging
• Spot/band cutting
• In gel or in solution digestion using trypsin or other enzymes

Infrastucture: 

• 4000 Q TRAP LC/MS/MS System which is a high performance hybrid triple quadrupole/linear ion trap LC/MS/MS mass spectrometer suitable for protein identification, post-translational modification detection and protein quantitation
• Agilent 1100 HPLC system for sample fractionation
• Voyager DE PRO MALDI TOF mass spectrometer for accurate molecular weight determination
• 2D electrophoresis system for various gel size
• Pharos FX plus gel documentation system with QuantityOne and PDQuest softwares for proper band and spot detection and comparison

Methods: 

• Protein quantitation using SILAC and iTRAQ methods
• Phosphoprotein enrichment and post-translational modification detection
• 1D and 2D electrophoresis

Contact: 

Éva Csősz, Ph.D. cseva[at]med.unideb.hu

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