Laboratory Animal Core Facility

Personal data: 

Attila Pap
Junior research fellow
E-mail: papa[at]med.unideb.hu
http://bmbi.med.unideb.hu

Services: 

The Laboratory Animal Core Facility was founded in 2005 to provide a professional background of animal experiments and promote best practices of human care, use and management of animals involved in medical research at the University of Debrecen. We advance the development and acceptance of high standards for the responsible use of laboratory animals and accurate and reproducible animal experiments.
The Laboratory Animal Core Facility is administratively responsible for all animal care resources and the purchase and care of all laboratory animals in the Medical and Health Science Center. The Core Facility operates a clinical pathology laboratory for the purpose of basic disease surveillance and diagnosis as well as serving as a research resource to investigation. Suitable housing conditions are provided for a wide variety of outbred, inbred and genetically modified laboratory animals as mice, rats, guinea pigs and invertebrate organisms.


MOUSE STRAINS

OUTBRED STRAINS
1. NMRI
INBRED STRAINS
1. Balb/C
2. C57BL/6
3. db/db
4. ob/ob
5. NOD
GENETICALLY MODIFIED STRAINS
1. AND
2. CD11c DTR
3. NOD CD1d KO
4. LysMCre
5. MXCre
6. CD11cCre
7. RunXCre
8. NUR77
9. PARP1 KO
10. PARP2 KO

11. PPARα KO
12. PPARγ floxed

13. RXRα floxed
14. RXRβ KO

15. TG2 KO
16. RXRα floxed RXRβ KO
17. PPARγ floxed LysMCre
18. PPARγ floxed MXCre
19. Dicer floxed
20. STAT1 KO
21. STAT6 KO
22. IL4 KO
23. BoyJ

RAT
Wistar white (on demand)

GUINEA PIG
Hartley (on demand)

ALTERNATIVE MODELS
C. elegans N2 strain
Biomphalaria glabrata (currently not available)

 

Infrastucture: 

The facility is located on the Debrecen University Campus as a part of Life Science Building.
Specialized animal housing spaces include a quarantine suite, infectious biohazard containment suites. All animal rooms and the operation rooms are ventilated with HEPA filtered air. Our technologies see the requirements of FELASA recommendations and DIN EN ISO 9001 standards. The animal rooms are ventilated with sterile filtered positive pressured (+10%) air. The exchange rate is 13.000 m3 air/hour (with tenfold ventilation). The sterility of air flow is tested regularly by sedimentation test and cultivation of sedimented bacteria.

Animals are housed separately by species and, when possible, by source and or microbiological status.The rodent colonies are housed and maintained under minimal disease (MD) and specified pathogen free (SPF) conditions. In our SPF definition our strains are negative for the following animal pathogens:

Methods: 

The laboratory capabilities include histology, immunocytochemistry, clinical chemistry, chromatography, bacteriology, genotyping (PCR), gene expression by RT-QPCR, flow cytometry, bone marrow transplantation, measurement of some metabolic parameters.

Contact: 

Attila Pap papa[at]med.unideb.hu

Photos: